Oh, wow, so much going on in this hilarious deviation. Here's what I think the conversation would be like:
: Good day, everypony, and welcome aboard Muffin Airways flight 666 to Manehattan, with a three-day stopover in Fillydelphia. Captain Screwball is your pilot and I am Derpy Hooves, your flight attendant, mechanic, medic, co-pilot, vice president and, um, a lot of other important things. Derp! We will take off as soon as I solder these two wires together with this bubble gum. Enjoy your flight.
: Why-why-WHY am I on this plane? I really need to practice with my own wings so I can fly on my own....
Spike: (sitting next to her) Well, Twilight, besides you being a worse flyer than Fluttershy, Manehattan's a gazillion furlongs away, you are being expected at a royal reception on the tarmac once we touch down, and this is the only flight out of Ponyville serving Manehattan.
: Oh boy! I LOVE flying Muffin Airways! Muffins are the only thing they serve on the menu, Derpy does EVERYthing on the plane, and it's really cool when they barrel over and fly upside down! BEST AIRLINE EVERRRR!
Spike: This airline's also got a great safety record. Best in all Equestria. Nopony's died on a Muffin flight yet.
: (shaking) We're gonna be the first! : Oh... my. Poor Twilight is so nervous. Eh.. excuse me, Twilight, is there anything I can do to calm you down? : Perhaps The Great and Powerful Trixie can provide assistance to Her Highness. Behold, Trixie shall come to Princess Twilight's aid and calm her nerves so that Her Highness can have some peace and rest!
(A mallet appears over Twilight's head and knocks her out)
Spike: Cool! Thanks, Trixie!
: You're welcome, my friend. (to herself) I've waited four seasons to do that.
: Thank you, Great and Powerful Trixie. She's our paramedic, fillies and gentlecolts.